Screening and Isolation of Bacteria Producing Biosurfactants from Waste

Biosurfactant are surface active compounds (SAC), amphiphilic in nature consisting of hydrophobic (long chain fatty acids) and hydrophilic domain (amino acid, protein, carbohydrate). Classification this compound is based on molecular weight, mode action physico-chemical structure. being utilized laundry formulation, house hold cleaning products, as antimicrobial agents, detergents, pesticides, food pharmaceutical industries. Various type microorganism has been studied capable synthesizing wide range biosurfactant by utilizing hydrocarbon source carbon energy. Present study aims to isolate producing bacteria from different waste sources. In among twenty-one isolated microbial cultures samples were screened for production using methods like hemolytic activity, blue gar assay, tolerance oil spread test, Emulsification index (E24%).